Small RNA Sequencing - Lexogen

Reagents & Kits

Small RNA Sequencing

The Small RNA-Seq Library Prep Kit for Illumina consists of all the reagents and components 
required to generate small RNA libraries to be used for Next Generation Sequencing on an Il-
lumina platform. All molecular reagents including adapters, primers, enzyme mixes, and buffers 
are provided. A column-based purification is included for rapid purification of nucleic acid prod-
ucts generated during the workflow.

•    Small RNA-Seq Library Prep Kit for Illumina
•    Modules & Add-ons 
 

Small RNA-Seq Library Prep Kit for Illumina

The Small RNA-Seq Library Prep Kit provides a protocol for generating small RNA libraries for Illumina sequencing, directly from total RNA or enriched small RNA.
It enables discovery and profiling of all common types of small RNA’s (≤ 200nt), including miRNA, piRNA, siRNA, snoRNA, tsRNA, srRNA and U-RNA
o    Gel-free user-friendly protocol
o    Wide input range from 50pg to 1µg
o    Optimized for low RNA-content samples such as plasma, serum and urine
o    Ready-to-sequence libraries in less than 5 hours
o    Multiplexing of up to 96 samples

Exceptional miRNA discovery
The protocol allows for detection of a higher number of microRNA’s compared to other workflows. The difference is especially considerable for lower RNA inputs

figure 1: Total number of miRNAs detected across 4 different protocols. Dilution series (6pg, 60pg and 600pg) of purified plasma RNA used for library prep from different vendors were performed. The obtained libraries were sequenced at equal molarity at 1.5-2M total raw reads per sample. The Lexogen kit showed much higher number of detected miRNAs at ≥ 5 Raw reads across all concentrations tested

Optimized for RNA from challenging Sources
The high sensitivity of the kit makes it the best choice for challenging, low content RNA sources, such as liquid biopsies (plasma, serum and urine), including exosomes. The protocol has also been tested on RNA from cells and tissues and can be used for inputs from 1ng-1µg of cellular total RNA or 50pg-1µg of enriched small RNA, including liquid biopsies.

High Reproducibility
The protocol exhibits exceptional reproducibility and correlation across samples with different concentrations.

figure 2: a: Dilution series of purified plasma RNA in small RNA-seq shows high inter-replicate correlation reads pr Million (RPM) across concentrations tested (60pg and 600pg) b: Dilution series of purified plasma RNA in small RNA-seq shows high correlation RPM across concentrations tested (60pg and 600pg). As and example, only data for 60pg and 600pg are shown

Gel-free User-friendly and Fast protocol
The Small RNA-Seq Library Prep Kit for Illumina offers a time saving protocol that can be completed within 5 hours and requires just 1h15’ of hands-on time. The final library does not need to go through gel purification. Quick and convenient magnetic bead-based purification can optionally be performed to remove adapter dimers for demanding RNA inputs.

Multiplexing of up to 96 samples
With the 96 i7 indices, standardly included in the kit, multiplexing of up to 96 samples is possible. This allows to pool more samples per sequencing lane and to perform cost-efficient experiments on the platforms of different scale.

Complete solution for Small RNA analysis
In order to get high quality input material for the Small RNA Library Prep, we recommend to extract the RNA with the SPLIT RNA extraction kit. This kit enables extracting NGS-grade total RNA or small and large RNA fractions from the same sample. SPLIT is highly suitable for enrichment of small RNA for use with the Small RNA-Seq Library Prep Kit.
 

Ordering information:
Contact us for pricing and additional information 

Cat. n°

Description

Reaction size

052.08

Small RNA-Seq Library Prep Kit for Illumina

8 preps

052.24

Small RNA-Seq Library Prep Kit for Illumina

24 preps

052.96

Small RNA-Seq Library Prep Kit for Illumina

96 preps

058.08

Small RNA-Seq Library Prep Kit for Illumina, including Purification Module with magnetic beads

8 preps

058.24

Small RNA-Seq Library Prep Kit for Illumina, including Purification Module with magnetic beads

24 preps

058.96

Small RNA-Seq Library Prep Kit for Illumina, including Purification Module with magnetic beads

96 preps

Modules and Add-ons for QuantSeq Kits

click here

 


SPLIT RNA Extraction Kit


The SPLIT RNA Extraction Kit enables a fast and highly efficient extraction of RNA that is free of genomic DNA contamination. The RNA can be recovered as total RNA or split into two fractions, large RNA and small RNA, facilitating the analysis of e.g., mRNA and miRNA from the same sample. The RNA obtained is ideal for seamlessly preparing libraries for Next Generation Sequencing of small RNA’s.

Ordering Information:

Cat. n°

Description

Reaction size

008.48

SPLIT RNA extraction Kit

48 rxn


Contact us for pricing and additional information 

Gel Extraction Module

The Gel Extraction Module enables the size selection of Small RNA-Seq libraries in a convenient spin column format. It prevents unwanted sequences (e.g., linker-linker artifacts and longer library fragments) dominating the NGS results and helps focus the sequencing depth on the desired microRNA fraction.

The module includes a reagent box consisting of Loading Dye, NGS MW Ladder, and NGS Control Ladder, and a purification box with all necessary buffers and purification columns for clean-up.

Ordering information:

Cat. n°

Description

Reaction size

054.24

Gel Extraction Module

24 rxn


Contact us for pricing and additional information

•    Purification Module with Magnetic beads 

The Purification Module with Magnetic Beads contains all the necessary reagents to carry out additional purifications.

Ordering information:

 

Cat. n°

Description

Reaction size

022.96

Purification Module with Magnetic beads

200 rxn


Contact us for pricing and additional information

LEARN MORE ABOUT SMALL RNA SEQUENCING

Request a quote or ask your question about Small RNA Sequencing via the contact options below. We will try to answer your question within one working day.

CONTACTFORM

Direct contact

+31 30 6880771
info@isogen-lifescience.com

OUR OFFICE

Veldzigt 2A
3454 PW De Meern

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