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Next to our Master Mixes, we also provide (the same) polymerases individually or together with other reagents in kits. See all of our polymerases listed below.
S7 Fusion Polymerase™ is a robust and a high-fidelity DNA polymerase that shows remarkable results in multiple PCR applications. Fusion of the polymerase to the Sso7d domain from Sulfolobus solfataricus, which has processivity enhancing capabilities, makes the S7 Fusion Polymerase™ one of the fastest and most robust PCR polymerases on the market. Is has a 3’ to 5’ exonuclease (proofreading) activity and creates blunt ends in PCR.
Comparison of S7 Fusion Polymerase to another high fidelity enzyme (P), both with GC and HF buffer, and a standard Taq polymerase. A 2756 bp PCR ampliqon was analyzed by PacBio sequencing and the percentage of PCR errors is displayed in the figure above. Using high fidelity polymerases result in less errors compared to using the standard Taq polymerase and using HF buffers show best performances.
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