PCR Cleanups

Reagents & Kits

PureIT ExoZAP PCR Cleanup

Amplified PCR products can cause troubles in downstream applications due to excess of primers, ssDNA and/or dNTPs still being present. Treating your amplified PCR products with PureIT ExoZAP PCR Cleanup, an enzymatic PCR clean up, will make your samples ready for applications such as sequencing.

Features PCR Cleanups

•    One-step PCR Cleanup 
•    Fast protocol, only 5 minutes
•    No need for spin columns or magnetic beads
•    Treatment improves downstream applications such as DNA sequencing and SNP analysis
•    Minimal loss of PCR products during treatment

 

PureIT ExoZAP

The PureIT ExoZAP PCR CleanUp is a one-step PCR cleanup consisting of two components; a heat labile Exonuclease (HL-Exo) l and a recombinant Shrimp Alkaline Phosphatase (rSAP). Together these enzymes will remove left over primers, ssDNA and will inactivate excess dNTPs by dephosphorylation. 
The PureIT ExoZAP PCR CleanUp can be pipetted directly into your amplified PCR product. Incubation at 37°C for 2 – 5 minutes is enough to accomplish this process. Followed by incubation at 80°C for 3 – 10 minutes will successfully deactivate both enzymes completely. 
The PCR products are now cleaned up and ready for further downstream applications (for example sequencing or SNP analysis).

Download brochure
 

PureIT ExoZAP Kit

The PureIT ExoZAP PCR Cleanup Kit has two separate components; a heat labile Exonuclease (HL-Exo) l and a recombinant Shrimp Alkaline Phosphatase (rSAP). In the correct ratio these two enzymes will remove left over primers, ssDNA and will inactivate excess dNTPs by dephosphorylation.
Both components of the PureIT ExoZAP PCR Cleanup can be pipetted directly into your amplified PCR product. Incubation at 37°C for 2 – 5 minutes is enough to accomplish this process. Followed by incubation at 80°C for 3 – 10 minutes will successfully deactivate both enzymes completely. 
The PCR products are now cleaned up and ready for further downstream applications (for example sequencing or SNP analysis). 

Download brochure
 

PureIT ExoZAP PCR CleanUp improves quality of DNA sequencing

Amplification of a 866 bp sized PCR product was performed using TEMPase DNA Polymerase in Ammonium Buffer. The PCR products was spiked with primers and dNTP’s followed by treatment (A) without PureIT ExoZAP PCR CleanUp (B) with PureIT ExoZAP PCR CleanUp (2 + 3 min) (C) with another enzymatic PCR clean-up reagent from a competitor (1 + 4 min). The samples were analyzed in triplicates and according to manufactures recommendations. The results of Sanger sequencing between de samples was compared by plotting the number of base calls in graphs showing a quality value score (Phred score). The PCR products that were treated with PureIT ExoZAP resulted in a significantly improved quality of DNA sequencing. Next to this, PureIT ExoZAP PCR CleanUp showed comparable PCR clean-up results to the competitor’s clean-up. *Data with Quality Values below 10 are not shown. 

The Sanger sequencing results of the 866 bp sized PCR product are displayed above. The upper electropherogram is after treatment with PureIT ExoZAP PCR CleanUp and the lower electropherogram is untreated. The displayed region is from 250 – 300 bases of the sequence. Treating the spiked PCR product with PureIT ExoZAP PCR CleanUp resulted in a significantly improved Sanger sequencing quality. Signal intensities are enhanced and background interference and base miscalls are eliminated.

LEARN MORE ABOUT PCR CLEANUP

Request a quote or ask your question about PCR CLEANUP via the contact options below. We will try to answer your question within one working day.

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