PCR Master Mixes

Name: Isogen Life Science B.V.
Address: Veldzigt 2a, De Meern, 3454 PW, NL
Telephone: +31 30 6880771

Save costs with our PCR Master Mixes

Master mixes help you to save time and reproducibility

Our ready to use Master Mixes are great alternative options to your regular individual PCR reagents. Master mixes are reagents that include all the components needed for a PCR. By only the extra step of adding your template and primer pairs you are able to run a PCR quickly and successfully.

Minimize possible contamination of the stock reagents due to fewer handling steps, enhancing the reproducibility of your experiments.
Reduce your experiments time.

Share your research, challenges and experiences with us so we can so we can discuss the best approach on how to proceed with your research.

We offer a broad range of master mixes. Have a look at the ones described below to choose which ones match better with your project needs.

Taq DNA Polymerase Master Mix RED
RealQ Plus PCR Master Mix
NEW! RealQ Fast 2x Master Mix Green
AQ97 High Fidelity DNA Polymerase Master Mix
Other Master Mixes

Each of our Master Mixes has its own benefits. All the master mixes specified below are available with different buffer options, giving wider options to finally chose the master mix that matches your PCR conditions and application.

Have a look at customer reviews!

PCR master mixes for your protocols

We want to help you on making your research happen.

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Taq DNA Polymerase Master Mix RED

Ready-to-use premixed solutions are perfect for your standard PCRs. Taq polymerases give steady and reliable results. All Master Mixes are 2x reaction mixtures and are available with a final MgCl2 concentration of 1.5 mM or 2.0 mM.

Reduce the extra step of adding loading buffers.

The color red of the master mix provides a perfect direct loading of your PCR products after amplification. It enables the visualization of the product when pipetting and gel loading.

This Master Mix consists of:

Taq DNA Polymerase
Ammonium (NH4+) buffer system
dNTPs
Magnesium chloride (MgCl2).

Next to this, it contains a red dye and a stabiliser, which do not affect PCR reaction. If desired for your protocol experiments, the red dye can be removed afterwards by using PureIT ExoZAP PCR CleanUp or by spinning the column purification. Moreover, with your clean PCR product, your sample can be used for sequencing.

Features:

Premixed solution
Direct loading on agarose and SDS DNA gels
Dye front runs at 300 – 1000 bp on a 0.5 – 1.5 % agarose gel
No need for a separate loading buffer
dUTP incorporation possible
Processes up to 5 kb
Leaves a 3’dA overhang

Benefits:

Easy visualization of pipetting and loading gels
Minimal optimization needed
Higher reproducibility due to less pipetting steps
High product yield

Our Taq DNA Polymerase Master Mix RED showed great performance and results when targeting targets different length genes: PAH (203 bp), Q8 (727 bp), CFTR (613 bp) and BAIP (788 bp).

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Applications: Genotyping using Q-Extract DNA Extraction PCR Kits

The AppNote shows the study performed with the Q-Extract DNA Extraction PCR Kits in combination with either one of the two available master mix: Taq DNA Polymerase 2x Master Mix RED or TEMPase Hot Start DNA Polymerase 2x Master Mix BLUE.

The extraction and amplification of genomic DNA was performed from five different mouse tissues (kidney, muscle,
lung, liver and tail), chicken muscle tissue and human saliva.

The results show that cam be used for many different mammalian samples and also saliva.
A wide range of sample amounts was analysed
Genotyping results were achieved in 70 to 210 minutes

Read to AppNote for more information about the use of the master mix.

Have a look at the AppNote

RealQ Plus PCR Master Mix

RealQ Plus PCR Master Mixes are ready-to-use 2x master mixes used for different Real-Time PCR applications. Real-Time PCR is nice technique that enables the quantification of DNA and the analysis of gene expression levels.
RealQ Plus master mixes consist of TEMPase Hot Start DNA Polymerase, dNTPs, MgCl2 and a special buffer system necessary to carry out high quality DNA amplification. Additionally, our master mixes have a high stability and efficiency when working with cDNA and gDNA.

To ensure you that RealQ Plus PCR Master Mix gives the same robust results time after time, we can proudly say that the Master Mix is produced under ISO 9001:2015 conditions. As a result, our master mix provides you reliable and robust results time after time.

Two variants are available in combination with three different ROX™ reference dye levels.

RealQ Plus Master Mix Green: DNA-binding fluorescent with a green dye for detection.
RealQ Plus Master Mix for Probe: it uses a detection based on probe real-time PCR.

Features

High specificity, reproducibility, efficiency and stability
Reaction set-up at room temperature
All-in-one premixed 2x solution

To select the correct ROX™ reference dye levels for your specific Real-Time PCR applications, check the “Real-Time instrument guide”. An advantage of our two variants of master mixes is that both can be chosen when many genes need to be analysed within a short amount of time.

ROX™ reference dye (without rox, with low or high rox)

Match your needs!

Stability studies with the qPCR master mix

Experiment: Two plates were prepared for a qPCR reaction, but were incubated afterwards in the dark at room temperature for 48 and 72 hr before the actual run.

Results: High stability and complete inactivation state of the TEMPase is seen before hot start. This allows researchers to run their plates at room temperature, avoid changes on temperature, and work on a more convenient time instead of directly after setting up the reaction.

NEW! RealQ Fast 2x Master Mix Green

Our latest solution, the RealQ Fast 2x Master Mix Green is a high-quality, convenient solution for dye-based real-time PCR workflows. With its optimized formulation, this ready-to-use master mix ensures sensitive, reliable, and rapid results, facilitated by the inclusion of 2Taq Hot Start DNA Polymerase.

Precise control over enzyme activity: Antibody-mediated hot-start mechanism of 2Taqprevents undesirable amplification at low temperatures, minimizing the risk of nonspecific amplification.
Versatile choice! It is compatible with a wide range of real-time PCR isntruments because of the three separate ROX solution available.

SENSITIVE DETECTION AND ACCURATE QUANTIFICATION

The RealQ Fast 2x Master Mix Green provides sensitive detection and quantification across a wide range of DNA templates including human genomic DNA, plasmid DNA and bacterial DNA.

More information and prices

AQ97 High Fidelity DNA Polymerase Master Mix

AQ97 High Fidelity DNA Polymerase Master Mix is a 2x Master Mix used in applications in which high fidelity is important like in cloning, next generation sequencing (NGS) applications and/or mutagenesis. It has the ability to amplify difficult DNA targets (with low or high GC content) as well as long DNA targets.

Features
• 2x all in one master mix
• Quick reaction set up
• High fidelity. .60 x Taq fidelity
• 3’ to 5’ proofreading exonuclease activity
• High elongation rate: 10sec/kb
• Long-range capability: 11kb gDNA
• Good amplification on DNA template with low to high GC content

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Distribution of Substitution errors

PCR was performed using Taq DNA Polymerase, AQ97 High Fidelity DNA Polymerase, high fidelity DNA Polymerase Q and high fidelity DNA Polymerase P.

The PCR was followed by NGS sequencing of the PCR products. The number of substitutions at each PCR target position was calculated and plotted in diagram A.

Substitutions include misincorporated nucleotides and deletions at each position. Non-polymerase errors are subtracted from the total number of errors to revel true polymerase errors. Non-polymerase errors include mutations caused by thermocycling-induced DNA damage, pre-NGS sample preparation and sequencing errors. In these diagrams the average number of substitutions for Taq DNA Polymerase (Taq average) and for AQ97 High Fidelity DNA Polymerase (AQ97 average) is also plotted.

Diagram B magnifies the area near the detection limit, displaying more information about the number of substitutions for AQ97 High Fidelity DNA Polymerase, high fidelity DNA Polymerase Q and high fidelity DNA polymerase P.

Download the Q97 High Fidelity Brochure

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Other Master Mixes

Besides the mentioned master mixes, we offer different solutions that might be interesting.

Taq Polymerase Master Mix
- Taq DNA Polymerase Master Mix
- Taq OptiMix CLEAR
TEMPase Hot Start DNA Polymerase Master Mix
- TEMPase Hot Start Master Mix
- TEMPase Hot Start Master Mix Blue
- Multiplex TEMPase Master Mix
GC TEMPase Master Mix
- GC TEMPase Master Mix l
- GC TEMPase Master Mix ll
- GC-rich DNA Target Kit

Is any of those catching your attention?

Customer review

"We switched to Taq DNA Polymerase Master Mix RED and Real Q Plus 2x Master Mix Green after comparison with similar products. Here they proved to be better or at least as good, had a higher user-friendliness and at a clearly lower cost per reaction. We are very happy with both products and would definetly recommmend them.

Tina Fuglsang Daugaard - Research laboratory technician Arthus University - DK

Aarhus University - Studenten UU - Students UU

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Why Isogen Life Science?

At Isogen Life Science, we believe that long term relationships with life science professionals and suppliers are fundamental to solve life science problems. Our curiosity drives us to continually stay involved and to dig deep in dialogue, with both clients and suppliers. It’s these intensive collaborations that enable us to develop tailored, innovative products that push life science’s boundaries. Partner up with us and enjoy our quick support for troubleshooting and questions!