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When you are looking for the best reagents and kits regarding labelling chemistry, such as reagents for Western blotting, antibody labelling or even HTRF (Homogenous Time Resolved Fluorescence) technology, you are at the right place!
We offer you products regarding all previously mentioned techniques produced by Cyanagen, Advansta and Bio-Nobile. Leading companies in the field of reagents for molecular biology techniques. Their products are based on patented technologies in which outstanding performance in sensitivity and stability can be expected.
Scroll down or click on the topics below to read more.
WESTAR is a product line for enhanced chemiluminescence (ECL) substrates for Western blotting applications. ECL is commonly used for Western blotting detection since it is an easy method with great sensitivity. The new WESTAR HYPERNOVA is the brightest HRP (Horseradish Peroxidase) substrate available. The signal intensity is extremely high and has a stable light output which makes it suitable for detecting trace protein levels. A low background results in an extremely high signal-to-noise ratio.
WESTAR HYPERNOVA - Cyanagen
GEHC – Amersham ECL Select
SuperSignal West Femto – Thermo Scientifi
High sensitivity of WESTAR HYPERNOVA compared to other substrates (GEHC-Amersham ECL Select & SuperSignal™ West Femto-Thermo Scientific™)
A) Detection of purified IκBα with WESTAR HYPERNOVA and two other substrates (1st Ab: Rabbit anti-IκBα, 1:1000, followed by 2nd Ab: Goat anti-rabbit, 1:500.000). A 1.5-fold dilution series of purified IκBα (from 667 pg to 5 pg) was imaged simultaneously with both substrates for 10 seconds with ImageQuant™ LAS 4000 (GE Healthcare).
B) Signal-to-noise ratio (S/N) analysis for all substrates. The enlarged inset shows the increased sensitivity of WESTAR SUPERNOVA substrate. LOD = 5 pg compared to LOD = 39 pg, SuperSignal™ West Femto (LOD = limit of detection).
Other available ECL substrate kits are:
Advansta’s WesternBright multi-colour fluorescent (MCF) Western blotting kit enables visualisation of two proteins at once, and thereby increasing the quality and quantity of information that can be gained from a single blot. Imaging a control protein alongside a protein of interest is possible with this kit. Just like imaging phosphorylated and unphosphorylated isoforms of a protein simultaneously. The fluorescent dyes provided with WesternBright MCF outperform Cy™ dyes and allow detection of low pg of protein. The WesternBright protocol saves time and money since there is no need for disposable film, and the blot can be imaged immediately, without drying.
Detection of phosphorylated and unphosphorylated isoforms of a protein simultaneously.
EGFR and phospho-EGFR can be imaged simultaneously using WesternBright MCF Western blotting kit. Chemiluminescence detection of the same experiment would require stripping and reprobing of the blot which can result into more errors and thereby influencing the outcome. A431 cell lysates (lane1) and A431 cells treated with EGF (lane 2) were blotted. EGFR is detected in green (b) and phospho-EGFR in red (c). An overlay picture is seen in (a). Molecular weight marker in lane 3.
WesternBright ECL spray is optimized for chemiluminescent Western blots imaged using film. The same components as Advansta’s WesternBright ECL link to webshop, but put together in one easy spray which makes measuring or mixing unnecessary. Simply spray onto the blot and you are ready to image.
WesternBright ECL is a very strong substrate which allows you to use up to 10x less antibody compared to other substrates. Due to the fewer steps needed, less pipettes, tips and tubes are used resulting in additional savings and reduced waste.
Next to this, we offer other Western blotting products as well, such as:
STAR FLUOR secondary antibodies are secondary antibodies of high quality. They are designed for use in fluorescent immunoassays such as flow cytometry, cell imaging and fluorescent Western blotting.
Detection of low protein amounts is possible due to the superior brightness of the antibodies where high signal-to-noise ratio is guaranteed.
Human Transferrin visualized by fluorescent Western blotting using Rabbit anti-human transferrin primary antibody and Goat anti-Rabbit Star Fluor550 (in red), Goat anti-Rabbit Star Fluor645 (in green). Proteins are imaged with ImageQUANT™ LAS 4000 ( GE Healthcare).
ELISTAR substrates is the patented product line of Cyanagen and specifically made for ELISA detection. ELISTAR HYPERNOVA is the brightest HRP ECL substrate on the market to date. The substrate is extremely sensitive and its light output is extraordinary, allowing detection of trace amounts of proteins. Low background levels result is a very high signal-to-noise ratio.
BioNobile’s QuickAllAssay™ kit covers all reagents needed for labelling proteins with europium chelate. The kit can be used for both homogenous and enhanced separation-based time-resolved fluorescence (TRF) assays.
TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer)
TR-FRET includes two fluorophore labels; Eu chelate donor and a fluorescent acceptor, in close proximity of each other. TR-FRET uses the long decay Eu donor to improve the FRET energy transfer. The acceptor is activated to emit light; usually between 570 – 740 nm.
Separation-based TReF (Time-Resolved enhanced Fluorescence assays)
A coated plate or another solid phase is needed to bind and separate one component of the assay. The bound Eu-complex fluorescence is measured after a dissociative enhancement. A second chelating reagent is added to recapture all Eu and reform a new highly fluorescent chelate.
qFM (Fluorescence Modulation) TRF
This assay only needs one labelled component, usually a Eu-chelate attached to a ligand. Both end point as well as kinetic assays can be measured with this technique. A proprietary assay buffer modulates any free chelate fluorescence enabling direct measurement of only the bound ligand-receptor or ligand-cell complex.
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