ATP Kits

Reagents & Kits

ATP Kits

All living cells contain ATP (adenosine triphosphate) and when the cell dies ATP is degraded by intracellular enzymes to ADP and finally AMP. The intracellular ATP concentration is similar in different cell types. Bacterial cells contain 1-2 attomoles per cell while animal and plant cells contain 100 to 10 000 times as much ATP. 
Extracellular ATP may be degraded by adding ATP consuming enzymes or by physical separation. Determination of intracellular ATP can then be used to estimate biomass. If the amount of ATP per cell is known the cell number may also be estimated. 
In sample which likely contains urine, extracellular ATP, ATP in somatic cells and ATP in bacterial cells all three ATP pools can be determined. Determination of bacterial ATP in urine is used to determine bacteriuria in the diagnosis of urinary tract infection.
 All ATP assays shall be calibrated by measuring the light before and after adding a known amount of ATP standard at the end of each test. This calibration obviates analytical interference from all sorts of variation of reagents and sample matrices and provides a result expressed in moles rather relative light units.

Intended use:

  • Determination of bacterial ATP in urine in the diagnosis of urinary tract infection (UTI)
  • Antibiotic susceptibility testing
  • Antibiotic concentration determination
  • Determination of somatic cells in cow milk (mastitis testing)
  • Tumor chemosensitivity testing
  • Cell proliferation and cytotoxicity

 

Quicklinks:

Intracellular ATP kit

  • For determination of intracellular ATP in bacterial cells
  • The sensitivity of this kit allows determination of low ATP levels in a variety of circumstances provided it is not important to degrade ATP in somatic cells:
  • Bacteriological control of liquids (e. g. drinking water, process water, beverages and beer)
  • Pharmaceuticals and cosmetics
  • Cell adhesion to surfaces
  • Quality assurance of laundry
  • Bacterial growth.
  • Antibiotic effects on bacteria
  • Article code: 266-111

Biomass ATP Kit

  • For determination of the total (both extracellular and intracellular) amount of ATP in a sample.
  • The ATP Biomass Kit is suitable for virtually all types of samples.
  • The sensitivity of this kit allows determination of low ATP levels in a variety of circumstances:
  • Liquids (e. g. drinking water, process water, environmental samples, beverages and beer)
  • Pharmaceuticals and cosmetics
  • Cell adhesion to surfaces (e. g. biocompatibility studies)
  • Quality assurance of laundry
  • Bacterial growth.
  • Antibiotic effects on bacteria
  • Article code: 266-311

Microbial ATP Kit

  • For determination of intracellular ATP in bacterial cells among mammalian cells
  • The sensitivity of this kit allows determination of low ATP levels in a variety of circumstances:
  • Bacteriological control of liquids (e. g. drinking water, process water, beverages and beer)
  • Pharmaceuticals and cosmetics
  • Cell adhesion to surfaces
  • Quality assurance of laundry
  • Bacterial growth.
  • Antibiotic effects on bacteria
  • Article code: 266-112
     
  • LEARN MORE ABOUT OUR ATP KITS
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