16S/16S-18S Microbiome analysis

Reagents & Kits

16S/16S-18S Microbiome analysis

Depending on your input source, we offer different kits and services.
When looking at the gut microbiome, the 16S Long Read kit or the 16S Read Cloud kit will be the most suited solution.
When working with environmental samples, such as soil or water, the 16S-18S kit will give the best results as this one has been optimized to work with low biomass samples.

Unlike other kits that mostly only target V3 and V4, the Loop Genomics technology enables targeting the entire 16S gene and therefore all 9 variable regions. This enables a far more accurate classification of species down to species level with great confidence.

With LoopSeq 16S and LoopSeq 16S-18S, the power of single molecule counting is combined to long-read sequencing, resulting in the lowes possible false positive rate and the best species level classification. 
The technology allows to sequence each 16S molecule V1-V9 in a sample and to phase each molecule.
In addition, the single-molecule counting technology eliminates PCR bias and reports molecules based on true abundance. This means that even molecules in very low abundance are detected and quantified, giving a true and accurate image of the species in your sample.

The LoopSeq technology enables over 90% of the reads being classified to species level, whereas with the common V3-V4 systems only allow 35-40% of the reads being classified to species level.

16S Profiling Kits and service

  • 16S Microbiome profiling from Mammalian Derived samples
  • Covering entire 16S rRNA gene and all 9 variable regions
  • >99% Unique 16S Molecules assigned at species or genus level
  • Accurate UMI Based quantification
  • 40x lower error rates than standard Illumina workflows

 

LoopSeq 16S Long Read Kit

  • Classification and quantification down to species level
  • Most comprehensive data

 

LoopSeq 16S Read Cloud Kit

  • Classification and quantification down to genus level
  • Lower sequencing depth required; more cost-effective alternative

 

Long-Read 16S rRNA Gene sequencing and analysis service

  • Service includes library prep, sequencing and Data analysis
  • Resulsts delivered as easy to understand reports and raw data files
  • Scalable pricing, depending on number of samples (minimal 8 samples)
  • Highly attractive volume pricing
     

16S-18S Profiling Kits and service

 

  • Microbiome profiling from Environmental samples (water, air, soil, surface swabs, …)
  • Compatible with low biomass samples
  • Identification of both prokaryotic and eukaryotic microbes
  • Long reads covering entire 16S (V1-V9) and 18S gene
  • Accurate UMI based quantification
  • 40x lower error rates than standard

 

LoopSeq 16S-18S Long Read Kit

  • Classification and quantification down to species level
  • Most comprehensive data

 

LoopSeq 16S-18S Read Cloud Kit

  • Classification and quantification down to genus level
  • Lower sequencing depth required; more cost-effective alternative

 

Long-Read 16S-18S Sequencing and Analyses service

  • Service includes library prep, sequencing and Data analysis
  • Results delivered as easy to understand reports and raw data files
  • Scalable pricing, depending on number of samples (minimal 8 samples)
  • Highly attractive volume pricing
     

Contact us for pricing of kits and service and additional information

Cat. n°

Description

reaction size

LG16S24RXNSSC

LoopSeq™ 16S Long Read Kit

24rxn

LG16S24RXNSSRC

LoopSeq™ 16S Read Cloud Kit

24rxn

LG16S18S24RXNSSC

LoopSeq™ 16S-18S Long Read Kit

24rxn

LG16S18S24RXNSSRC

LoopSeq™ 16S-18S Read Cloud Kit

24rxn

LEARN MORE ABOUT 16S/16S-18S MICROBIOME ANALYSIS

Request a quote or ask your question about the 16S/16S-18S Microbiome analysisour Plate Readers via the contact options below. We will try to answer your question within one working day.

CONTACTFORM

Direct contact

+31 30 6880771
info@isogen-lifescience.com

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