PureIT ExoZAP PCR CleanUp 2500R 1 tube x 0.5 mL

PRODUCT CODEA620606

PRODUCT DESCRIPTION

PureIT ExoZAP PCR CleanUp 2500 R 1 tube x 0.5 mL

Why do I need this?

If you plan to sequence your PCR product, you must remove all residual PCR primers and unincorporated nucleotides. Sequencing uses one primer, while PCR utilizes two. If we try to sequence with two primers present, you’ll get the two sequences back, superimposed on each other and completely unreadable.1

FEATURES

One-step PCR Clean-up for optimal sequencing results. Clean-up results in just 15 minutes.

  • One-step PCR product clean up
  • Fast 15 min protocol
  • No need for spin columns or magnetic beads
  • Treatment improves downstream applications such as DNA sequencing and SNP analysis
  • Minimal loss of PCR products during treatment

DESCRIPTION

PureIT ExoZAP PCR CleanUp consists of a balanced combination of a heat labile Exonuclease (HL-ExoI) I and a recombinant Shrimp Alkaline Phosphatase (rSAP).

Treatment of amplified PCR products with PureIT ExoZAP PCR CleanUp, removes residual primers, single-stranded DNA and inactivates excess dNTPs by dephosphorylation. Add PureIT ExoZAP PCR CleanUp directly to the reaction containing the amplified PCR product. After treatment at 37 °C for 5 minutes PureIT ExoZAP PCR CleanUp is completely inactivated by heating at 80 °C for 10 minutes.

PROTOCOL

This protocol serves as a guideline for clean-up of 5 μl PCR product using PureIT ExoZAP PCR CleanUp*.

  • Take out PureIT ExoZAP PCR CleanUp from the -20 °C freezer.
  • Keep PureIT ExoZAP PCR CleanUp on ice at all times.
  • Add 2 μl PureIT ExoZAP PCR CleanUp** to 5 μl of amplified PCR product.
  • Incubate the reaction at 37 °C for 5 minutes to degrade remaining primers, single-stranded DNA and to inactivate excess nucleotides by dephosphorylation.
  • Incubate at 80 °C to completely inactivate PureIT ExoZAP PCR CleanUp.
  • The cleaned up PCR product can now be used for downstream applications such DNA sequencing, primer extension experiment or SNP analysis.
  • After treatment the PCR products can be stored at -20 °C

*If treating PCR product of higher volume, then increase proportionally the amount of PureIT ExoZAP PCR CleanUp.

**PureIT ExoZAP PCR CleanUp works in PCR buffers.