Ampliqon Taq DNA Polymerase is ideal for routine PCR applications requiring high yield and also reliable DNA amplification.
Ampliqon Taq DNA Polymerase has a molecular weight of 95 kDa and exhibits both a 5'→3' DNA polymerase and a 5'→3 exonuclease activity. The 5'-3' exonuclease activity leaves a 3'dA overhang on the PCR product, which are convenient for direct T-A cloning. Taq DNA Polymerase lacks 3'-5' exonuclease activity and proofreading ability.
Taq DNA polymerase is suitable for standard testing, routine PCR, screening and high throughput testing.
Ampliqon Taq DNA Polymerase was compared with a Taq DNA Polymerase from a well-recognised competitor. Three different DNA target varying in length PAH (203 bp), ENG9 (293 bp) and ENG 5 (480 bp) were evaluated. Ampliqon Taq DNA Polymerase performed equally well or better in this study. Furthermore, Ampliqon Taq DNA Polymerase displays a higher specificity on the PAH and ENG5 targets, than the competing Taq DNA Polymerase. Each Amplification has been conducted according to supplier's manual.