Taq DNA Polymerase 5 U/ul 500U

PRODUCT CODEA111103

PRODUCT DESCRIPTION

Taq DNA Polymerase 5 U/ul 500U, 10x Ammonium Buffer and 25 mM MgCl2 1 tube x 0.1 mL + 2 tubes x 1.5 mL

Ampliqon Taq DNA Polymerase is ideal for routine PCR applications requiring high yield and also reliable DNA amplification.


FEATURES

  • A thermostable recombinant DNA Polymerase
  • High product yield
  • dUTP incorporation possible
  • processes up to 5 kb
  • Leaves a 3'dA overhang


DESCRIPTION

Ampliqon Taq DNA Polymerase has a molecular weight of 95 kDa and exhibits both a 5'→3' DNA polymerase and a 5'→3 exonuclease activity. The 5'-3' exonuclease activity  leaves a 3'dA overhang on the PCR product, which are convenient for direct T-A cloning. Taq DNA Polymerase lacks 3'-5' exonuclease activity and proofreading ability.

Taq DNA polymerase is suitable for standard testing, routine PCR, screening and high throughput testing.

 

COMPARISON BETWEEN AMPLIQON TAQ DNA POLYMERASE AND A COMPETITOR 

Ampliqon Taq DNA Polymerase was compared with a Taq DNA Polymerase from a well-recognised competitor. Three different DNA target varying in length PAH (203 bp), ENG9 (293 bp) and ENG 5 (480 bp) were evaluated. Ampliqon Taq DNA Polymerase performed equally well or better in this study. Furthermore, Ampliqon Taq DNA Polymerase displays a higher specificity on the PAH and ENG5 targets, than the competing Taq DNA Polymerase. Each Amplification has been conducted according to supplier's manual.

 

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