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BMG Labtech Application Notes
Low-volume Absorbance Microplate/Cuvette Reader
The SPECTROstar Nano represents a revolution in absorbance measurement instrumentation with the flexibility to perform assays quickly and easily in both microplates or via the built-in cuvette port. Using a spectrometer, this new absorbance microplate reader captures a full UV-Visible spectrum (220 to 1000 nm) in less than 1 sec/well. The speed of the spectrometer, simple push button operation, and the capacity to design and save individual assay protocols give users an unmatched flexibility to optimize settings for all absorbance experiments. In combination with the LVis
Plate sample volumes down to 2 µL can be measures with a maximum of 16 samples in parallel.
The SPECTROstar Nano also includes the following outstanding features
- Ultra-fast UV/Vis absorbance spectrometer
- Captures a spectrum from 220 to 1000 nm in less than 1 s/well
- Selectable spectral resolution of 1 - 10 nm
- 6- to 1536-well microplate format compatibility
- Low volume 2 µl measurements for DNA, RNA, protein etc.
- Standard cuvette port for individual samples
- Incubation up to 45 °C for microplate and cuvette
- Gas vent for atmosphere sensitive samples
- Well scanning, kinetic and endpoint measurements
- Multimode plate shaking capabilities
- Assay specific quick run protocols
- Powerful Data Analysis Software - MARS
- FDA 21 CFR part 11 compliant
- Robot compatibility
- Much more...
The SPECTROstar Nano is delivered with a new version of Reader Control software and comprehensive Data Analysis Software, MARS.
With its simple to use 'Quick Run' feature it reads ELISAs, RNA, DNA, Protein (Bradford, BCA, Lowry), Cell growth, and Beta-galactosidase assays at the touch of a button due to predefined protocols.
This highly intuitive software also provides limitless possibilities for test set up where it is needed. In MARS, a wizard guides the user through the entire data analysing process. Additionally, a template manager can be used for building complex data processing protocols.
Improved robotic integration capabilities, digital signature and FDA 21 CFR part 11 compliance of MARS complete this powerful software package.
|Detection modes: || Absorbance UV/Vis |
|Measurement modes: || Endpoint and Kinetic |
|Microplate Formats: || 6 to 1536-well plates, user-definable |
|Microplate Carrier: || Robot compatible |
|Cuvette port: || Cuvette port for cuvettes with 10 mm path length |
Beam height 8.5 mm
|Light source: || High energy xenon flashlamp |
|Detector: || Spectrometer with CCD |
|Wavelength range: || 220 - 1000nm |
|Sensitivity (ABS): || Full spectrum captured in < 1 s/well |
Selectable spectral resolution: 1, 2, 5, 10 nm
OD range: 0 to 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Reproducibility: ±0.005 OD for 0 - 2 OD range
|Reading Speed (96-well): || Full spectrum from 220 to 1000 nm in less than 1 sec/well |
|Shaking: || Linear, orbital, and double-orbital with user-definable time and speed |
|Gas vent: || System to inject an atmosphere or to pull a vacuum into the reader |
|Incubation: || +4 °C above ambient up to 45 °C |
|Optics: || Diode Array |
|Special: || Cuvette port for standard |
and low volume cuvettes,
volume down to 2 ul
|Upgrade: || LVisplate for microliter measurements |