CLARIOstar Plus - Most flexible Plate Reader for Assay Development
CLARIOstar Plus - Most flexible Plate Reader for Assay Development
CLARIOstar Plus - Most flexible Plate Reader for Assay Development
CLARIOstar Plus - Most flexible Plate Reader for Assay Development
CLARIOstar Plus - Most flexible Plate Reader for Assay Development
CLARIOstar Plus - Most flexible Plate Reader for Assay Development

CLARIOstar Plus - Most flexible Plate Reader for Assay Development

PRODUCT CODE430-501

PRODUCT DESCRIPTION

The new CLARIOstar® Plus is the latest addition to our family. Our most flexible multi-mode plate reader comes equipped with our patented LVF Monochromators™, filters, and spectrometer. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development.

The CLARIOstar Plus is a multi-mode microplate reader with advanced LVF Monochromators™, highly sensitive filters, and an ultra-fast UV/vis spectrometer. The Enhanced Dynamic Range and automatic focus technologies make manual settings superfluous and detection optimisation easier than ever. 

Atmospheric Control Unit, temperature incubation, multiple shaking, well scan and reagent injectors make the CLARIOstar Plus the ideal platform for any live cell-based assay.

Application Notes

Technical specifications

Detection modes

Fluorescence Intensity including FRET
Fluorescence Polarization/Anisotropy
AlphaScreen®/AlphaLISA®/AlphaPlex™
Luminescence(flash and glow) including BRET
Time-Resolved Fluorescence including TR-FRET
UV/Vis Absorbance Spectra

Measurement modes

Top and Bottom reading
Endpoint and Kinetic measurements
Sequential Multi Excitation measurements
Sequential Multi Emission measurements
Spectral Scanning (fluorescence, luminescence, absorbance)
Ratiometric measurements
Well Scanning

Microplate Formats

6- to 1536-well plates, user-definable
LVis Plate with 16 low volume microspots (2 µl)

Microplate carrier

Robot compatible

Light sources

High energy xenon flash lamp
Dedicated Laser for AlphaScreen® / AlphaLISA® / AlphaPlex™

Detectors

Low noise photomultiplier tube
Read-shifted photomultiplier tube
CCD spectrometer

Wavelength selection

Dual Linear Variable Filter (LVF) Monochromators™
Linear Variable Dichroic Mirror
Separate excitation and emission LVF Monochromators
Optical Filters:
  • Excitation and emission slides hold 4 filters each

  • LVF Monochromators + optical filters: use one for excitation and the other for emission

  • UV/Vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well

Optical Filters

Excitation and emission slides for 4 filters each

Optical Path Guides

Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics

Z-Adjustment

Automatic focal height adjustment (0.1 mm resolution)

Spectral Range

Filters:
FI, FP, TRF: 240 - 740 nm or 240 - 900 nm (red-shifted PMT)
LUM: 240 - 740 nm

LVF Monochromators™
FI: 320 - 740 nm or 320 - 840 nm (red-shifted PMT)
LUM: 320 - 740 nm

Linear Variable Dichroic: 340 - 740 nm or 340 - 760 nm (red-shifted PMT)

Spectrometer: ABS: 220 - 1000 nm

Sensitivity

FI filters:
top: < 0.15 pM (< 3 amol/well FITC, 384sv, 20 μL)
bottom: < 1.0 pM (< 50 amol/well FITC, 384g, 50 μL)

FI monochromator:
top: < 0.35 pM (< 7 amol/well FITC, 384sv, 20 μL)
bottom: < 3.0 pM (< 150 amol/well FITC, 384g, 50 μL), dynamic range: 8 decades in a single measurement

FP: < 0.5 mP SD at 1 nM FITC (384sv, 20 μL)

HTRF®: (black and white microplates) Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 880 % (High Calibrator)
Delta F > 30 % (Low Calibrator)

TRF: < 20 fM europium, 384, 80 μL
LUM: < 0.4 pM (< 8 amol/well ATP, 384sv, 20 μL), dynamic range: 8 decades in a single measurement

AlphaScreen® with laser: < 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL)

ABS with spectrometer:
Full spectrum captured in < 1 s / well
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 - 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD

Read times

Flying mode (1 flash): 8 s (96), 15 s (384), 28 s (1536)
10 flashes: 19 s (96), 57 s (384), 3 min 4 s (1536)

Reagent Injection

Up to 2 built-in reagent injectors
Individual injection volumes for each well: 3 to 500 µL (optionally up to 2 mL)
Variable injection speed up to 420 µL / s
Reagent back flushing

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Integrated Barcode Reader

Up to two integrated barcode readers

Incubation

+3 °C above ambient up to 45 °C or 65 °C
The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.

Software

Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
Integrated fluorophore library

Dimensions

Width: 45 cm, depth: 51 cm, height: 40 cm; weight: 32 kg

Optional Accessories

LVis Plate

Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples.
Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates.

Atmospheric Control Unit (ACU)

Actively regulates O2 and CO2 - 0.1 - 20%

Stacker

Magazines for up to 50 plates – continuous loading feature

THERMOstar

Microplate incubator and shaker

Optical Filters

Excitation and emission filters

Upgrades

The CLARIOstar Plus can include all or any combination of features listed below at purchase. Upgrading with additional features is possible at any time. Please contact your local representative for more details or a quote.


LOD = 3 x SD (20 blanks) / slope (6 pt std curve), AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C, Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS, 96 = 96-well microplates, 384sv = 384-well small volume microplates, 384g = 384-well glass bottom microplates, 1536 = 1536-well microplates

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