Isogen Highlights March 2018 - PCR special

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Isogen Highlights March 2018 - PCR special

PCR plastics

Isogen Life Science offers a full range of high quality plastics for PCR including PCR tubes, PCR strips and well plates.

Our team knows how important your PCR reactions are and how long takes to set up your protocol to have reproducible and good results. This is why we want to provide you good quality plastics for any protocol or system that you have in your lab.

All our plastic for PCR are Certified RNase-, DNase- and DNA- and Endotoxin free and manufactured to strict QC standards with ultra-thin walls to ensure rapid thermal transfer. Standard pack size: 1000 tubes per pack.

PCR tubes

We offer 2 different volumes: 0.2 or 0.5 ml. Available in natural color or mixed (mixed packs contain blue, green, red, violet and yellow tubes) with domed or flat caps.

If you want to have extra-clear plastic, this option is also available for your important qPCR reactions in 0.2 ml tubes.

If you have problems with contamination, the integral shield on the cap can help to prevent finger contamination with the surface of the lid.

PCR strips

8 - PCR Strips (Individually Attached Caps), 0.2 ml and 0.1 ml

  • Polypropylene PCR strips
  • Individually attached caps eliminates the possibility of cross-contamination through accidental cap-tube mix ups
  • Angled lids allow the tubes to be nested together in racks without interfering with one another
  • Supplied in resealable bags
  • Certified RNase, DNase, DNA and PCR Inhibitor free
  • Domed caps, or extra clear flat caps for qPCR
  • Available as standard height (20.8 mm) or low-profile (15.2 mm) tubes
  • Mixed pack contains blue, green red, violet and yellow strips (24 of each)
  • Pack sizes: 120 strips or 5 x 24 strips

PCR Strips (Attached, Cap Strip)

8-Strip PCR Tubes, Attached Domed Cap Strip

  • Attached, hinged strip caps
  • Supplied in resealable bags
  • 0.2 ml. Pack sizes: 125 strips or 5 x 25 strips
8-Strip PCR Tubes, Attached Flat Cap Strip

  • Available with opaque, white tube with extra-clear caps for qPCR
  • Extra clear caps combined with natural tubes or white tubes
  • 0.2 ml. Pack size: 125 strips
8-Strip Non-Flex PCR Tubes, Individually Attached Caps

Non-flex tubes have a robust, triple link between each tube making them less flexible and easier to handle. These tubes are made by the latest 'twin shot' technology which enables a white tube and an optically clear cap to be made in the same mould. Ideal for qPCR, white tubes maximise reflectance for enhanced signal and the extra clear caps allow optimal signal transmission and detection.

  • Available with low-profile (15.2 mm) or standard height (20.8 mm) tubes
  • Pack sizes: 120 strips
  • 0.1 or 0.2 ml volume available
8-Strip PCR Tubes, Individually Attached Shielded Caps

  • Each domed cap has a shield to help prevent accidental finger contamination during opening.
  • 0.2 ml volume, 120 strips per pack and natural color.

PCR Strips (Separate Tubes & Caps)

8-Strip PCR Tubes and Caps

  • Tube and cap strips are sold separately
  • Natural or mixed colour available
  • Opaque white tubes for qPCR
  • Extra clear flat cap strips for qPCR
  • 'Perfect Seal' cap strips for easier attachment to plates or tube strips without affecting tightness
  • 0.2 ml. Pack size: 125 strips, 5 x 125 strips, 5 x 25 strips.
12-Strip PCR Tubes and Caps

  • Tube and cap strips are sold separately
  • Natural or mixed colour available
  • Extra clear flat cap strips for qPCR
  • ‘Perfect Seal’ cap strips for easier attachment to plates or tube strips without affecting tightness
  • 0.2 ml. Pack size: 80 strips, 5 x 16 strips

4-Strip Rotor-Gene® Style Tubes & Caps

  • 0.1 ml 4-strip PCR tubes and caps
  • suitable for use with the Rotor-Gene® Q Real-Time PCR Cycler
  • Pack size: 250 tube strips and 250 caps strips.

PCR Well Plates

Not all plates are manufactured with the same plastics, accuracy and quality to ensure good results. Why is it important ? What can happen to the results?

Isogen Life Science offers a full range of well plates depending on your needs and instrument.

Why are our well plates perfect for you?

How to know which well plate fits perfectly with my cycler system?

 

 

  • Manufactured from the highest quality grade of polypropylene
  • Rigorous QC measures ensure uniformity of thin walls without any twist of the plate when your protocol ends
  • Opaque white or black plates show low auto-fluorescence with chemiluminescent (white) or fluorescence (black)
  • Manufactured to strict QC standards with ultra-thin walls to ensure rapid thermal transfer.
  • Highly visible contrasting printed matrix

PCR Plates, Non-Skirted

  • Cuttable plate available! Can easily be cut with scissors to any desired format. More pliable than other non-skirted plates so it can easily be cut into any desired configuration
  • Maximum capacity: 350 µl (200 µl low-profile plates), 96 wells
  • Can be sealed with the sealing formats: strip caps, or plate seals suitable for PCR.
  • LOW PROFILE available

Silicone Sealing Mat

Reusable, ultra-flexible silicone sealing mat for 96-well PCR plates.
Designed to be especially suitable for thermal cyclers with pressure-fit lids.
Can be cut to size for use with 24, 32 or 48-well plates.
The non-reactive rubber will withstand repeated autoclaving, extreme temperatures and aggressive chemicals.

PCR Plates, Non-Skirted, Elevated Wells

  • Elevated wells help prevent cross-contamination
  • Highly visible contrasting printed matrix
  • Available in 96-, 48- or 24-well format
  • Moulded matrix available

PCR Plates, Semi-Skirted

  • Maximum capacity: 350 µl (200 µl low-profile plates), 96 wells
  • Natural color or black
  • Low profile and Straight Edges available
  • PCR Plate for Roche® Lightcycler® available (white)
  • Semi-Skirted PCR Plate with Raised Rim and Raised Rim, low profile for FAST Systems

PCR Plates, Skirted

  • Holes on side for robotics handling
  • Natural, white or black color available
  • Silicone Sealing Mat fits to ensure non evaporation
  • 96 wells

PCR Plates, 384-Well

  • Holes on sides for robotics applications
  • 384-Well Sealing Mat
  • Options:
    1. 384-Well PCR Plates, ABI® / Universal-Style
    2. 384-Well PCR Plate for Roche® Lightcycler®
    3. 384-Well PCR Plates (35 µl)

PureIT ExoZAP

Buy online:
A620601 PureIT ExoZAP PCR CleanUp 100R 1 tube x 0.5 mL Log in to see the price Buy
Buy online:
A620606 PureIT ExoZAP PCR CleanUp 2500R 1 tube x 0.5 mL Log in to see the price Buy
Buy online:
A620603 PureIT ExoZAP PCR CleanUp 500R 1 tube x 0.5 mL Log in to see the price Buy
Buy online:
A620607 PureIT ExoZAP PCR CleanUp 5000R 1 tube x 0.5 mL Log in to see the price Buy

Why do I need this?

If you plan to sequence your PCR product, you must remove all residual PCR primers and unincorporated nucleotides. Sequencing uses one primer, while PCR utilizes two. If we try to sequence with two primers present, you’ll get the two sequences back, superimposed on each other and completely unreadable.1

FEATURES

One-step PCR Clean-up for optimal sequencing results. Clean-up results in just 15 minutes.

  • One-step PCR product clean up
  • Fast 15 min protocol
  • No need for spin columns or magnetic beads
  • Treatment improves downstream applications such as DNA sequencing and SNP analysis
  • Minimal loss of PCR products during treatment

DESCRIPTION

PureIT ExoZAP PCR CleanUp consists of a balanced combination of a heat labile Exonuclease (HL-ExoI) I and a recombinant Shrimp Alkaline Phosphatase (rSAP).

Treatment of amplified PCR products with PureIT ExoZAP PCR CleanUp, removes residual primers, single-stranded DNA and inactivates excess dNTPs by dephosphorylation. Add PureIT ExoZAP PCR CleanUp directly to the reaction containing the amplified PCR product. After treatment at 37 °C for 5 minutes PureIT ExoZAP PCR CleanUp is completely inactivated by heating at 80 °C for 10 minutes.

PROTOCOL

This protocol serves as a guideline for clean-up of 5 μl PCR product using PureIT ExoZAP PCR CleanUp*.

  • Take out PureIT ExoZAP PCR CleanUp from the -20 °C freezer.
  • Keep PureIT ExoZAP PCR CleanUp on ice at all times.
  • Add 2 μl PureIT ExoZAP PCR CleanUp** to 5 μl of amplified PCR product.
  • Incubate the reaction at 37 °C for 5 minutes to degrade remaining primers, single-stranded DNA and to inactivate excess nucleotides by dephosphorylation.
  • Incubate at 80 °C to completely inactivate PureIT ExoZAP PCR CleanUp.
  • The cleaned up PCR product can now be used for downstream applications such DNA sequencing, primer extension experiment or SNP analysis.
  • After treatment the PCR products can be stored at -20 °C

*If treating PCR product of higher volume, then increase proportionally the amount of PureIT ExoZAP PCR CleanUp.

**PureIT ExoZAP PCR CleanUp works in PCR buffers.

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Brilliant labels

Dual labelled probes in 10 nmol scale!

For a high yield you don’t need a high synthesis scale!

Isogen Life Science provides high yields for all synthesis scales. Therefore you don't need to order high scales.

Order online

Comparison with other competitors of average yields for a 20-mer salt free oligo in a 10 nmol synthesis scale
Brand Synthesis scale (µmol) Yield (OD)
ISOGEN 0.010 8
Supplier 1 0.025 3
Supplier 2 0.025 2
Supplier 3 0.025 3
Supplier 4 0.020 3

With our high yields, we can provide excellent dual-labelled probes in a 10 nmol synthesis scale, with HPLC purification included!

Please find our competitive prices below:
Dual labeled probes
5' and 3' label combinations 10 nmol scale 50 nmol scale
5': FAM, TET, ATTO 520, JOE, HEX, Cy3, TAMRA,
ATTO 565, ROX, Texas Red, Cy3.5, ATTO 610,
Cy5, Cy5.5 3': Dabcyl, Eclipse, TAMRA, BHQ1,
BHQ2, BBQ 650
€ 129 € 175

5': Yakima Yellow, ATTO 480, ATTO 550, ATTO 633, ATTO 647N, ATTO 680,
DY-549, DY-610, DY-649
3': Dabcyl, Eclipse, TAMRA, BHQ1, BHQ2, BBQ 650
€ 209 € 255

Please contact us for more information: support@isogen-lifescience.com

Masterful Mastermix

Amplidiag™ Hot-Start PCR Master Mix is a high-performance ready-to-use solution for various needs. It has been optimized for high sensitivity and specificity in both multiplex and singleplex PCR and qPCR reactions.
Amplidiag™ Hot-Start PCR Master Mix comes with Mobidiag’s proprietary Taq DNA polymerase and utilizes antibody-based hot-start technology with very low residual activity before the initial denaturation step, thus increasing the specificity of the PCR.

Buy online:
AD-MM-101 Amplidiag 2x Master mix,1ml Log in to see the price Buy

 

SUBJECTS

Equipment