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Application notes

BMG Labtech Application Notes

CLARIOstar® - New High Performance Multimode Microplate Reader with Advanced LVF Monochromator™

The CLARIOstar is a multimode microplate reader with Advanced LVF Monochromators, highly sensitive filters, and an ultra-fast spectrometer. It is a modular microplate reader, having up to eight different detection modes, including: Fluorescence Intensity, FRET, Fluorescence Polarization, Luminescence, BRET, UV/Vis Absorbance, Time-Resolved Fluorescence, TR-FRET, and AlphaScreen® /AphaLISA®.

Besides the LVF Monochromators, the CLARIOstar can measure fluorescence and luminescence assays, including FRET and BRET, with fixed filters. Additionally, filters can be combined with the monochromators. Excite a fluorophore with a narrow bandwidth, high transmission filter and measure the emission with a broad bandwidth on the monochromator, or perform an emission spectral scan while exciting with a filter. Having filters that can be combined with a monochromator guarantees there is no compromise in performance in any fluorescence or luminescence assay. Moreover, all of these options are available to measure assays from both the top and bottom of the microplate.

In addition to filters and monochromators, the CLARIOstar incorporates an ultra-fast spectrometer for UV / Vis absorbance detection. This technology, pioneered several years ago by BMG LABTECH, allows users to spectrally scan an absorbance assay in less than 1 second per well. Users obtain an entire spectrum from 220 to 1000 nm at a 1 nm resolution; that is 780 distinct wavelengths in the same time other instruments measure only one or two. Full spectrum absorbance allows for assays not possible on other instruments.

Other technological features in the CLARIOstar ensure the best performance in advanced assay modes. AlphaScreen® and AlphaLISA® assays use a dedicated high energy laser, and fluorescence polarization, time-resolved fluorescence and TR-FRET assays use dedicated optical components. For bottom reading and cell based assays, direct optics are used, not fiber optics. Direct optics have a significantly higher light transmission, thereby allowing sensitive assays like fluorescent proteins to be easily measured, even with the LVF monochromator.

With the CLARIOstar, BMG LABTECH has created the perfect multimode microplate reader. Anything is possible - any wavelength, any bandwidth, and any assay.


Detection modes: Fluorescence Intensity including FRET
Fluorescence Polarization/Anisotropy
Luminescence(flash and glow) including BRET
Time-Resolved Fluorescence including TR-FRET
UV/Vis Absorbance Spectra
Measurement modes: Top and Bottom reading
Endpoint and Kinetic measurements
Sequential Multi Excitation measurements
Sequential Multi Emission measurements
Spectral Scanning
Ratiometric measurements
Well Scanning
Certification: HTRF®
Transcreener® far red FP
Transcreener® red FI
Transcreener® red TR-FRET
Microplate Formats: 6- to 1536-well plates, user-definable
LVis Plate with 16 low volume microspots (2 µl)
Light source: High energy xenon flash lamp
Dedicated Laser for AlphaScreen® / AlphaLISA®
Detector: Low noise photomultiplier tube
CCD spectrometer
Wavelength selection: Dual Linear Variable Filter (LVF) Monochromators™
Linear Variable Dichroic Mirror
Optical Filters
LVF Monochromators + Optical Filters
UV/Vis Absorbance Spectrometer
Full spectra or 8 distinct wavelengths in < 1 sec/well
Wavelength Range:


240-750 nm or 240-900 nm for FI, FP, TRF, 240-750 nm for LUM

LVF Monochromators

320-850 nm for FI, 320-750 nm for LUM

Linear Variable Dichroic

340-740 nm for FI, LUM


220-1000 nm for ABS
Sensitivity (FI): Filters (top): < 0.4 pM (< 8 amol/well Fluorescein, 384sv, 20 µL)
Monochromator (top): < 0.5 pM (< 10 amol/well Fluorescein, 384sv, 20 µL)
Filters (bottom): < 1.0 pM (< 50 amol/well Fluorescein, 384g, 50 µL)
Sensitivity (FP): < 0.7 mP SD at 1 nM Fluorescein (384sv, 20 µL)
Sensitivity (HTRF): Reader Control Kit (Eu) after 18h (384sv, 20 µL)
Delta F > 880 % (High Calibrator)
Delta F > 30 % (Low Calibrator)
Sensitivity (TRF): < 40 fM (< 0.8 amol/well Europium, 384sv, 20 µL)
Sensitivity (LUM): < 0.4 pM (< 8 amol/well ATP, 384sv, 20 µL)
Sentitivity (AplhaScreen®): < 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 µL)
Sensitivity (ABS): Full spectrum captured in < 1 s / well
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 - 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Reading Speed (96-well): 1 flash: 8 s
10 flashes: 19 s
Reading Speed (384-well): 1 flash: 15 s
10 flashes: 57 s
Reading Speed (1536-well): 1 flash: 28 s
10 flashes: 3 min 4 s
Shaking: Linear, orbital, and double-orbital with user-definable time and speed
Incubation: +3°C above ambient up to 45°C or 65°C