Cellometer® automatic cell counting principle is based on imaging cytometry. Cells in suspension are spread into a thin layer of liquid, as in a traditional hemacytometer. Cellometer® Cell Counters, connected to a computer via USB cable acquire cell image from multiple locations of a Cellometer® disposable hemacytometer. Cellometer® software automatically analyzes acquired cell images and measures cell concentration and cell size. Viability and live cell concentration are measured using trypan blue exclusion dye.

Cell Counting Steps: 1, 2, 3

Step 1. Pipette 20 micro-liter sample into a disposable Cellometer® chamber
Step 2. Place Cellometer® chamber into a cell counter
Step 3. Run Cellometer® software to obtain cell concentration and viability automatically
Typical cell line takes less than 30 second per sample.

The Cellometer Auto models are best suited for:

• Total cell concentration for highly viable cell population
• Live cell concentration and viability using trypan blue
• Cell size measurement
• Identify and count cells based on cell size difference
• Capture and store cell images

Features

• Disposable counting chamber
• Trypan blue viability
• Live image
• Estimate cell size
• Saves date & cell images

Benefits

• Simple & fast
• No extra reagent and processing
• Morphology observation
• Small footprint and low cost
• No washing and clogging

• High quality microscopic cell images for observation of cell morphology and sample quality
• Fast, accurate cell counting: less than 30 seconds for a typical cell line sample
• Declustering ability for clumpy cells
• Cell size histogram
• Review counted cells on screen for data verification
• Direct link to Nexcelom applications support team
• Auto saving for cell counting data and cell images for record keeping
• Ability to store and print data through computer network
• Sample adjustment calculation for the next step in the experiment to eliminate manual data transfer induced errors

• Deep gap counting chamber with innovative counting method for cells or spheres larger than 100 micron
• Option to manually adjust cell count
• Use default cell parameter for simple cell counting
• Import cell types from Nexcelom cell library (250+ cell types)
• Cell type wizard for automatic set up new cell types
• Modify cell size parameters when needed and re-calculate for new results
• Auto sample ID increment for large numbers of samples
• Detailed data for each cell count saved in Excel for comparison between samples

	Compact, Simple to Use Cellometer Auto takes up almost no bench space and is incredibly easy to use. It's pre-configured for hundreds of cell types, and configuring for new cell types is just as easy. Because it works with your PC or laptop, you get a full screen display, easy data input, and the ability to import/export data to a local or network location. Cellometer also runs on Intel based Macs running Parallels/WinXP.
	Fast Results Because it can generate cell counts, size measurements, and concentration calculations in approximately 30 seconds, Cellometer enables you to generate data many times faster than manual counting.
	Minimal Sample Prep Disposable Counting Chambers is the only consumable needed to run Cellometer. Only 20uL of sample is required, and the chambers load in seconds, and when done can be disposed, eliminating the need for cover slips and washing between samples.
	Trypan Blue Viability Trypan Blue treated cells are automatically counted as live or dead, and the percentage viability is automatically calculated. Live counted cells are indicated by a green circle on-screen, and Dead counted cells by a red-circle.
	On-screen Imaging No two cells are the same, which is why live cell morphology can be immediately viewed on-screen, and counted cells are indicated on-screen for further analysis. The counting results are indicated for each cell and images can be saved or exported for a secure record of all of your cell counts.
	Cell Size Measurement Unlike manual counting, Cellometer measures both individual and mean cell sizes, and can generate size distribution histograms for all counted cells. Multiple histograms can be overlaid for determining changes in cell sizes over time.
	Advanced Imaging Technology Microscope quality optics and advanced vision software ensure that even the most challenging samples can be counted. This includes counting specific cells in a heterogeneous sample, declustering cell clumps (such as MCF7), counting irregular shaped cells, and excluding cellular debris.
	Save & Export Data, Images, and Graphs Keeping records of your research is critical. With the click of a button, Cellometer instantly saves counting results, cell images, graphs, and data to a clipboard, local or network location.

Documentation

Brochure