Cellometer AutoX4 is a line of simple to use, automated cell counters with fluorescence detection. It combines brightfield microscopy with fluorescence to identify selected cells. The user can switch fluorescence optics modules to detect a wide range of fluorophores with excitation wavelengths from UV to deep red.
The Cellometer X4 is best suited for:
- Total cell concentration for highly viable cell population
- Live cell concentration and viability using trypan blue
- Cell size measurement
- Identify and count cells based on cell size difference
- Capture and store cell images
- Total nucleated cell concentration without lysing non-nucleated cells
- Live nucleated cell concentration without lysing non-nucleated cells
- Fluorescence based live cell concentration and viability for cell lines
- AO, PI, EB, DAPI, Hoechst
In addition to what is available with the Cellometer Auto line of cell counters, Cellometer Auto X4 has the following enhanced functionalities and capabilities:
- Brightfield (BR) and fluorescent(FL) image capabilities
- Brightfield assays for total cell count or trypan blue viability
- Fluorescence assays of DNA staining dye based cell counting for nucleated cells
- Brightfield and fluorescence combination assays for DNA staining dye or Esterase substrat based viability assays
- User switchable fluorescence optics modules for different dyes
- Software pre-installed for most common assays
Available Fluorescence Optics Modules:
Fluorescence Optics Module XB-535-401
Excitation / Emission : 470nm / 535nm
Example Fluorophores:
- Acridine Orange (+DNA)
- Calcein
- Fluorescein
- SYTO® 9
- SYTO® 13
Fluorescence Optics Module XB-595-501
Excitation / Emission : 527nm / 595nm
Example Fluorophores:
- Propidium Iodide
- Ethidium Bromide
- Rhodamine B
- SYTOX® Orange
Fluorescence Optics Module XB-450-301
Excitation / Emission : 375nm / 450nm
Example Fluorophores:
- DAPI
- Hoechst 33342 & 33258
Fluorescence Optics Module XB-695-601
Excitation / Emission: 630nm / 695nm
Example Fluorophores:
Specifications
| Fluorescence |
yes |
| Brightfield |
yes |
|