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Home > Cell counting

Nexcelom Cellometer Auto X4

Cellometer AutoX4 is a line of simple to use, automated cell counters with fluorescence detection. It combines brightfield microscopy with fluorescence to identify selected cells. The user can switch fluorescence optics modules to detect a wide range of fluorophores with excitation wavelengths from UV to deep red.

The Cellometer X4 is best suited for:

  • Total cell concentration for highly viable cell population
  • Live cell concentration and viability using trypan blue
  • Cell size measurement
  • Identify and count cells based on cell size difference
  • Capture and store cell images
  • Total nucleated cell concentration without lysing non-nucleated cells
  • Live nucleated cell concentration without lysing non-nucleated cells
  • Fluorescence based live cell concentration and viability for cell lines
  • AO, PI, EB, DAPI, Hoechst

In addition to what is available with the Cellometer Auto line of cell counters, Cellometer Auto X4 has the following enhanced functionalities and capabilities:

  • Brightfield (BR) and fluorescent(FL) image capabilities
  • Brightfield assays for total cell count or trypan blue viability
  • Fluorescence assays of DNA staining dye based cell counting for nucleated cells
  • Brightfield and fluorescence combination assays for DNA staining dye or Esterase substrat based viability assays
  • User switchable fluorescence optics modules for different dyes
  • Software pre-installed for most common assays

Available Fluorescence Optics Modules:

Fluorescence Optics Module XB-535-401
Excitation / Emission : 470nm / 535nm

Example Fluorophores:
  • Acridine Orange (+DNA)
  • Calcein
  • Fluorescein
  • SYTO® 9
  • SYTO® 13
Fluorescence Optics Module XB-595-501
Excitation / Emission : 527nm / 595nm

Example Fluorophores:
  • Propidium Iodide
  • Ethidium Bromide
  • Rhodamine B
  • SYTOX® Orange
Fluorescence Optics Module XB-450-301
Excitation / Emission : 375nm / 450nm

Example Fluorophores:
  • DAPI
  • Hoechst 33342 & 33258
Fluorescence Optics Module XB-695-601
Excitation / Emission: 630nm / 695nm

Example Fluorophores:
  • Allophycocyanin (APC)

 

Specifications
Fluorescence yes
Brightfield yes

 


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