LavaPep is designed for the accurate and sensitive determination of peptides.

LavaPep is based on Fluorotechnics proprietary fluorophore epicocconone which fluoresces intensely red on binding to proteins ( Coghlan et al, 2005 ). Binding is reversible rendering stained peptide completely amenable to mass spectrometry, N-term sequencing and to functional assays.

LavaPep is robust to substances that inhibit most peptide assays and is particularly suited to proteomic applications where downstream MS-compatibility is important. LavaPep provides the accuracy of AAA for peptide quantification at a fraction of the cost.

PRODUCT FEATURES

• Sensitive, enabling as little as 100 ng/mL peptide to be accurately quantified
• Wide range of convenient assay volumes: 100 µL - 3 mL
• Wide dynamic range, over 3-orders of magnitude
• Easy, simply mix 1 part working solution with 1 part of sample
• Quick - data can be read within 60 mins
• Assay is robust to interfering compounds such as DNA and solubilisation reagents used in 1-D and 2-DGE sample preparation
• Signal is not affected by light or temperature and remains stable for up to 6 hours
• Time-consuming heating and reduction steps of peptide are not required
• The kit is free of strong solvents and acids
• The kit is designed for use in a wide range of fluorescence measuring instruments
• Peptides are not precipitated or denatured and can be used in subsequent assays
• Ideal for high throughput

LavaPep Peptide Quantification Kit, 2000 samples LP-022010 add to cart

 

Specifications

Use	peptide quantification

 

Documentation

Brochure