FluoroProfile is designed for the accurate and sensitive determination of proteins.

FluoroProfile is based on Fluorotechnics proprietary fluorophore epicocconone which fluoresces intensely red on binding to proteins (Coghlan et al, 2005). Binding is reversible rendering stained protein completely amenable to mass spectrometry, N-term sequencing and to functional assays.

FluoroProfile is robust to substances that inhibit most protein assays and is particularly suited to quantifying proteins in samples prepared for 2-D and 1-D electrophoresis, IEF gels and anywhere accurate quantification of protein is needed.

PRODUCT FEATURES

• Sensitive, enabling as little as 40 ng/mL protein to be accurately quantified
• Wide range of convenient assay volumes: 100 µL - 3 mL
• Wide dynamic range, over 3-orders of magnitude
• Easy, simply mix 1 part working solution with 1 part of sample
• Convenient packaging - use FluoroProfile straight from fridge
• Quick - data can be read within 20 mins
• Assay is robust to interfering compounds such as DNA and solubilisation reagents used in 1-D and 2-DGE sample preparation
• Signal is not affected by light or temperature and remains stable for up to 6 hours
• Time-consuming heating and reduction steps of protein are not required
• The kit is free of strong solvents and acids
• Low protein-protein variability
• The kit is designed for use in a wide range of fluorescence measuring instruments
• Proteins are not precipitated or denatured and can be used in subsequent assays

Documentation

Brochure