Proteomics

Proteomics laboratories that run 2D gels have invested a great deal (time, expertise and money) into producing the sample, running it on a gel, staining and imaging to identify their proteins of interest. Many customers will go no further than image analysis and searching external databases to identify their proteins of interest based on pI and mw.

However, at some point the scientist will need to identify the protein spot/feature of interest. This generally entails excising the protein spot from the gel, digesting with a protease (usually trypsin) and using MS or MS/MS mass spectrometry to identify the peptide fingerprint of the protein, or amino acid peptide sequence of one of the peptides. The mass spectra are then submitted to protein databases for identification.

Product		use
	ProPrep LC	Protein digestion High-Throughput LC/MS sample preparation
	ProPrep II	in-gel protein digestion in-solution protein digestion direct spotting onto MALDI targets or LC vials
	ProPic II	Imaging and picking
	ProPrep	Digestion and MS-preparation
	ProGest	Protein digestion
	IMA transfer plate

2D gel electrophoresis
	Hoefer E2D Elite	2D electrophoresis

Protein dyes
	FluoroProfile protein quantification kit	protein quantification
	LavaBlue protein stain kit	protein staining
	LavaCell Live imaging stain	cell staining
	LavaPep - peptide quantification kit	peptide quantification
	LavaDigest protease monitoring kit	protease monitoring
	LavaPurple total protein blot stain	protein blot staining

ProPrep LC

Reliable Automation of Protein Digestion for LC and LC-MS

ProPrep LC

Sample prep made easy

When it comes to reliable automation of in-gel and in-solution protein digestion followed by sample preparation for mass spectrometric analysis, the choice is simple. Digilab designed the ProPrep LC specifically for this purpose, offering you peace of mind and giving your samples the best treatment.

The ProPrep LC comes standard with one reaction block, but this can be modified to include up to four independently controlled reaction blocks. You can also upgrade the ProPrep LC to a ProPrep II, which allows samples to be spotted to commercially available MALDI plates and LC vials.

ProPrep II Features:

Flow-through reaction blocks, 96 well format, temperature controlled
Reagent, chilling
Multi-enzyme use
Accurate liquid handling
In-gel or in-solution
Transfer to LC vials
Sample spitting
Customizable protocols
Ability to run multiple protocols
Fully enclosed & HEPA filtered

Downloads

Datasheet

Publications

Videos
- Protein Digestion
- Sample Desalting

Specifications
Use	Protein digestion
Use	High-Throughput
Use	LC/MS sample preparation

ProPrep II

Sample prep made easy

Reliable Automation of Protein Digestion - Take Control of Your Samples

If you are looking for the only way to reliably automate the preparation of your protein samples for downstream mass spectrometric analysis, then the ProPrep II is your solution. It offers protein digestion and processing for MALDI targets or LC/MS, automating all steps of sample preparation in a flexible manner, supported by pre-set or easily customized protocols. The ProPrep II offers you peace of mind and gives your samples the best treatment.

ProPrep II Features: