Proteomics
Proteomics laboratories that run 2D gels have invested a great deal (time, expertise and money) into producing the sample, running it on a gel, staining and imaging to identify their proteins of interest. Many customers will go no further than image analysis and searching external databases to identify their proteins of interest based on pI and mw.
However, at some point the scientist will need to identify the protein spot/feature of interest. This generally entails excising the protein spot from the gel, digesting with a protease (usually trypsin) and using MS or MS/MS mass spectrometry to identify the peptide fingerprint of the protein, or amino acid peptide sequence of one of the peptides. The mass spectra are then submitted to protein databases for identification.
ProPrep LC
Reliable Automation of Protein Digestion for LC and LC-MS


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When it comes to reliable automation of in-gel and in-solution protein digestion followed by sample preparation for mass spectrometric analysis, the choice is simple. Digilab designed the ProPrep LC specifically for this purpose, offering you peace of mind and giving your samples the best treatment.
The ProPrep LC comes standard with one reaction block, but this can be modified to include up to four independently controlled reaction blocks. You can also upgrade the ProPrep LC to a ProPrep II, which allows samples to be spotted to commercially available MALDI plates and LC vials.
ProPrep II Features:
- Flow-through reaction blocks, 96 well format, temperature controlled
- Reagent, chilling
- Multi-enzyme use
- Accurate liquid handling
- In-gel or in-solution
- Transfer to LC vials
- Sample spitting
- Customizable protocols
- Ability to run multiple protocols
- Fully enclosed & HEPA filtered
Downloads
Datasheet
Publications
Videos
- Protein Digestion
- Sample Desalting |
Specifications
| Use |
Protein digestion |
| Use |
High-Throughput |
| Use |
LC/MS sample preparation |
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ProPrep II


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Reliable Automation of Protein Digestion - Take Control of Your Samples
If you are looking for the only way to reliably automate the preparation of your protein samples for downstream mass spectrometric analysis, then the ProPrep II is your solution. It offers protein digestion and processing for MALDI targets or LC/MS, automating all steps of sample preparation in a flexible manner, supported by pre-set or easily customized protocols. The ProPrep II offers you peace of mind and gives your samples the best treatment.
ProPrep II Features:
- Flow-through reaction blocks, 96 well format,
temperature controlled
- Reagent, chilling
- Multi-enzyme use
- Accurate liquid handling
- In-gel or in-solution
- Spotting to MALDI targets, accommodating all models
- Transfer to LC, offline and online (optional)
- Customizable protocols
- Ability to run multiple protocols
- Fully enclosed & HEPA filtered
- Sample spitting
Don't need to spot to MALDI targets? Learn how the new ProPrep LC could be your ideal solution.
Downloads
Technical Brochure
Publications
Videos
- Protein Digestion
- Sample Desalting
- MALDI Target Spotting |
Specifications
| Use |
in-gel protein digestion |
| Use |
in-solution protein digestion |
| Use |
direct spotting onto MALDI targets or LC vials |
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