Proteomics

Proteomics laboratories that run 2D gels have invested a great deal (time, expertise and money) into producing the sample, running it on a gel, staining and imaging to identify their proteins of interest. Many customers will go no further than image analysis and searching external databases to identify their proteins of interest based on pI and mw.

However, at some point the scientist will need to identify the protein spot/feature of interest. This generally entails excising the protein spot from the gel, digesting with a protease (usually trypsin) and using MS or MS/MS mass spectrometry to identify the peptide fingerprint of the protein, or amino acid peptide sequence of one of the peptides. The mass spectra are then submitted to protein databases for identification.

Product		use
	ProPic II	Imaging and picking
	ProPic	Spot picking and imaging
	ProPrep	Digestion and MS-preparation
	ProGest	Protein digestion
2D gel electrophoresis
	Hoefer E2D Elite	2D electrophoresis
Protein dyes
	FluoroProfile protein quantification kit	protein quantification
	LavaBlue protein stain kit	protein staining
	LavaCell Live imaging stain	cell staining
	LavaPep - peptide quantification kit	peptide quantification
	LavaDigest protease monitoring kit	protease monitoring
	LavaPurple total protein blot stain	protein blot staining