BMG LABTECH NEPHELOstar -Isogen Life Science

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BMG LABTECH NEPHELOstar

The Unique Laser-Based Microplate Nephelometer

The NEPHELOstar microplate nephelometer detects particles in liquid samples by measuring forward scattered light when a laser beam is directed through the solution. This scattered light is detected at angles of up to 80°, making it approximately thirty times more sensitive than tradional transmission readers that measure the reduction in direct light passing through a sample well.

Flexibily and performance of the NEPHELOstar allow more applications to be adaped to microplate-based laser nephelometry than ever before:

Automated drug solubility screening
Microbial growth kinetics monitoring
Precipitation and polymerization monitoring

For BMG LABTECH application notes that can be performed on the NEPHELOstar, please click here.

The intensity and beam diameter of the laser light can be adjusted in the easy to use software, and this allows measurement of light scatter in colored or turbid solutions. In addition, the flexible beam diameter allows measurement in 24, 48, 96 and 384-well microplate format.

These features, along with BMG LABTECH´s Digital Photon Integration (DPI) technology and a detection angle of 80°, ensure that the NEPHELOstar is approximately thirty times more sensitive than conventional transmission readers.

Temperature-regulated heating plates above and below the entire plate movement area provide uniform incubation up to 38°C.

Developed to meet high-throughput demands, this microplate-based nephelometer is faster than conventional solubility detection methods with the highest sensitivity and flexibility.

Self-monitoring laser diode

The light source is a red laser with a wavelength range of 635 nm. A laser diode has the advantage of higher stability than other light sources which gives better precision. The laser intensity can be adjusted via the software.

Variable laser beam width

An exclusive feature of the NEPHELOstar: the ability to adjust laser beam width. With a narrow beam width, liquid surface effects (meniscus effects) are minimized.

Reads 384-well plate formats

The NEPHELOstar is the only microplate nephelometer with the ability to read 384-well plates with excellent sensitivity. Use less sample and produce more data per plate.

Full-cone angle detection system

With the most advanced detection system, the NEPHELOstar can integrate the largest scattering angle, up to 80°.

Specifications
Measurement modes	Endpoint and kinetic measurement Drug solubility testing Bacterial and fungal growth Quality control Quantification of macromolecules Formation of immunocomplexes
Light source	Self-monitoring laser diode Wavelength 635±10 nm Stability <0.2% deviation Lifetime 20,000 hours Output: 1 mW Selectable beam width: 1.5-3.5 mm Selectable intensity 0-100% Detects up to 80° full cone angle
Detector	Side-window, current type photomultiplier tube
Gain Control	Software selectable gain 1:4000 in 256 steps Automatic gain adjustment
Reagent Injection	Up to three built-in reagent injectors Injection into the well at measurement position Individual injection volumes for each well Injection volumes down to 3 µL Reagent back flushing
Shaking	Orbital and linear; programmable shaking time and width
Temperature Control	Temperature range from +5°C above ambient to 38°C Temperature stability 0.2°C
Sensitivity	Depends on particle size and liquid properties Silica detection (particle size 0.5 to 10 µm) 800 nM Dynamic Range: 5 decades Maximum measurement value (1s= 100,000 Relative Nephelometry Units)
Microplate Formats	Up to 384-well plates
Reading Speed (96-well)	23 s
Reading Speed (384-well)	66 s
Detection modes	Nephelometry