PHERAstar FS - The Next-Generation HTS Microplate Reader
The PHERAstar FS is the ultimate multidetection microplate reader for HTS and assay development, especially where speed and sensitivity are imperative.
The compact and versatile PHERAstar FS microplate reader is able to perform all leading non-isotopic detection technologies. Important features include:
- Tandem Technology - Spectrometer / filter based detection
- Ultra fast UV/Vis spectrometer for full absorbance spectra
- Fluorescence intensity, TRF, FP, Luminescence
- Dedicated UV-laser for TR-FRET measurements
- Advanced HTRF® performance
- AlphaScreen® laser
- On-board reagent injection for precise kinetic measurements
- Advanced top and bottom reading
- High-resolution cell layer scanning
- All new MARS data analysis software
Thanks to the unique modular optical system, sequential dual excitation, simultaneous dual emission, and ratiometric calculations, such as for FRET and BRET, the PHERAstar FS performs all assays with ease and speed. BMG LABTECH offers assay optimized and barcoded optic modules for your applications with appropriate filters, mirrors, beamsplitters, dichroics or polarizers incorporated into one hand-held module. Once inserted, the PHERAstar FS automatically selects the proper module for your assay protocol. Never worry about what dichroic, aperture, or filter is installed in your microplate reader.
No detection mode is compromised to accommodate the multifunctionality of the PHERAstar FS. The PHERAstar FS can read 6-1536 well plates with uncompromised sensitivity, - no need for special apertures or optics. Assay flexibility is further enhanced by precise temperature control to 45°C as well as multi-mode shaking capabilities. Automatic Z-height, gain adjustment, and plate mapping as well as the ability to export data in ASCII format complete the package.
The Stacker II is designed for integration with the PHERAstar FS minimizing microplate handling. It provides loading, unloading, restacking and continuous feeding of up to 50 microplates.
The PHERAstar FS comes with a new version of BMG LABTECH's well established Reader Control and a completely new MARS Data Analysis Software with significant ease-of-use enhancements such as S/N, Z' and many other standard calculations. The Wizard creates an easy step-by-step calculation of a standard curve and the Template Manager is an excellent tool for building complex data processing protocols. Thus, user-defined data analysis is done simply with one click of the mouse. Improved robotic integration capabilities, digital signature and the 21 CFR part 11 compliance of MARS complete this new software.
The PHERAstar FS has all of the features that one would expect from the ultimate HTS microplate reader in order to support nearly any application that you can imagine.
Applications include:
- Protein-protein interactions
- Genotyping
- Molecular binding assays
- Receptor ligand binding
- DNA quantification
- Enzyme activity and kinetic assays
- Cell based assays
- Reporter gene assays
- Protein quantification
- G-coupled protein receptors (GPCR)
- And many more assays
Specifications
| Detection modes |
UV/Vis Absorbance Spectra
Fluorescence Intensity - including FRET
Fluorescence Polarization
Time-Resolved Fluorescence - including TR-FRET
High-End AlphaScreen®
Luminescence - including BRET |
| Measurement modes |
Top and bottom reading
Endpoint measurements
Kinetic measurements
Sequential Dual Excitation measurements
Simultaneous Dual Emission measurements
Real-time ratiometric measurements |
| Microplate Formats |
6 to 1536-well plates, user-definable |
| Light source |
High energy xenon flashlamp
Nitrogen Laser for TRF and TR-FRET
Solid state Laser for AlphaScreen® |
| Detector |
Two matched pairs of photomultiplier tubes, optimized for different detection modes |
| Optic modules |
Up to six application specific optic modules built in |
| Z-Adjustment |
Automated focal height adjustment (0.1 mm resolution) |
| Wavelength range |
230 - 750 nm for FI, FP, LUM
230 - 900 nm for TRF
220 - 1000 nm for ABS |
| Sensitivity (FI) |
top: 0.4 pM sodium fluorescein (8 amol / well) (384)
2.0 pM sodium fluorescein (16 amol / well) (1536) |
| Sensitivity (FI) |
bottom:5.0 pM sodium fluorescein (0.2 fmol / well) (384) |
| Sensitivity (FP) |
1.0 mP SD at 1 nM sodium fluorescein (384)
3.0 mP SD at 1 nM sodium fluorescein (1536) |
| Sensitivity (TRF) |
25 fM europium (0.5 amol / well) (384)
100 fM europium (0.8 amol / well) (1536) |
| Sensitivity (HTRF) |
Reader Control Kit (Eu / Tb)
ΔF 1050% (Eu) and 1150% (Tb) (high calibrator)
ΔF 95% (Eu) and 110% (Tb) (low calibrator) |
| Sensitivity (LUM) |
10 amol / well ATP (384) |
| Sentitivity (AplhaScreen®) |
< 100 amol / well* (384) |
| Sensitivity (ABS) |
Spectral range: 220 - 1000 nm
Full spectrum captured in < 1 s / well
Selectable spectral resolution: 1 - 10 nm
OD range: 0 - 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD |
| Reading Speed (1536-well) |
1 flash: 27 s
10 flashes: 1 min 52s
50 flashes: 5 min 18s |
| Reading Speed (384-well) |
1 flash: 14 s
10 flashes: 38s
50 flashes: 1 min 29s |
| Reagent Injection |
Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 300 µl)
Variable injection speed up to 420 µl / s |
| Shaking |
Linear, orbital, and double-orbital
User-definable time and speed |
| Barcode reader |
Up to three integrated barcode readers |
| Temperature Control |
Incubation up to 45°C |
| Plate Capacity |
Magazines for up to 50 plates
Continuous loading feature |
|
Brochure