High Performance Size Exclusion Chromatography Media for Preparative and Process Scale Separation of Proteins
- Made From Agarose, Well Established and Well-known in the Biotech Industry
- Excellent Resolution
- Robust Separation Results Can be Achieved Across a Wide Range of Proteins & Conditions
- Chemically Stable for Cleaning in Place
WorkBeads™40 SEC Gel Filtration Media is designed for high performance protein separations under a variety of conditions. The high resolution that can be obtained makes ideal for both preparative work and process scale separation of proteins.
WorkBeads™40 SEC for Size Exclusion Chromatography or Gel Filtration Media has a high selectivity which means the protein peaks are well separated with greater distance from each other than comparable products made from synthetic polymers. This means that the media has capacity to separate proteins well even when using high proteins loadings.
Figure 1. shows the Kav curve for some common proteins. The dimer of thyroglobulin elutes in Vo and the other proteins nicely follow the theoretical Kav curve.
Resolution is the combined effect of selectivity (distance between peaks) and efficiency (peak width, depending on particle size). Separation media based on agarose are well known for excellent selectivity. The narrow particle size distribution around 40 micrometer in combination with the proprietary cross linking results a media that is easy to pack in columns with very high efficiency and good flow characteristics.
Specifications
| Particle size |
40 µm |
| Exclusion limit |
1200 kD |
| Type |
Size exclusion |
|
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