WorkBeads 40 ACT -Isogen Life Science

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WorkBeads 40 ACT

Activated Media for Preparative and Bioprocess Scale Affinity Chromatography with User’s Choice of Ligands

Made from Agarose, Well Established and Well-known in the Biotechnology Industry
Simple Coupling Procedures at Room Temperature
Stable at Room Temperature in Aqueous Solution and at Neutral pH
Suitable for Coupling of Ligands Containing Sulphydryl, Amino or Hydroxyl Groups

WorkBeads 40 ACT pre-activated separation media are produced from agarose using a proprietary cross linking method that result in a highly porous and physically stable agarose matrix. Agarose based matrices have been successfully used for decades in biotechnology research and in the industrial purification of proteins. Agarose is proven to be exceptionally compatible with natural bio-molecules such as proteins, DNA, carbohydrates etc. The material shows minimal non specific interaction due to the hydrophilic nature of agarose. Unlike matrices made from synthetic polymers, agarose does not have micro pores that can contribute to local pH variations in the micro-environment in the column which lead to distorted separations.

WorksBeads 40 ACT is activated according to the Bromohydrin method. This activation method is proprietary and based upon well known chemistry and allows you to perform the coupling chemistry in aqueous solutions.

WorkBeads 40 ACT is supplied as an aqueous suspension with 22% ethanol as preservative. After washing, the gel is immediately ready for use. As no toxic chemicals are involved and the WorkBeads 40 ACT products are stable at room temperature the coupling procedure can, as long as your application so allows, easily be performed on your bench and at room temperature.

Proteins or other molecules with free amino and sulfhydryl groups will couple. Just add the ligand to the suspension, stir and incubate over night. The media is ready for use. Hydroxy groups could also be used for coupling but will require pH above 12 which is not compatible with most proteins. However, stable molecules could be coupled using the hydroxyl group. Remaining reactive groups are deactivated using mercapto-ethanol or ethanol-amine.