ATP detection

The core technology of BioThema AB is analytical applications of the luminescent reaction of the Firefly Luciferase enzyme.
We provide luminescence assay reagents for monitoring of the Luciferase enzyme and ATP.

The standard in luminescence technology

BioThema has a unique ATP Standard that is stabilized in liquid to ensure accuracy of each luminometer reading. Calibration with internal Standard ATP accounts for variations between luminometers, inhibition or quenching of light from the sample and sensitivity of reagents.

ATP assay with ATP Reagent HS and Internal ATP Standard

1. Add 0.1 ml extractant B/S to a cuvette.
2. Add 0.1 ml sample to the cuvette.
3. Add 0.4 ml reconstituted ATP Reagent HS and measure light emission, I_smp.
4. Add 0.01 ml 10^-7 mol/l ATP Standard, i.e. 1 pmol ATP, and measure light emission, I_smp+std.

Calculations

Calculate amount of ATP (pmol) in the sample by the equation:

ATP_smp = 1 * I_smp / ( I_{smp + std} - I_smp)

The factor 1 is the amount of ATP Standard added (i.e. 1 pmol).

Notes

1. The higher the total assay volume the higher the sensitivity of the assay and the more accurate the results
2. The (I_{smp + std} - I_smp) value should be at least 10x higher than the I_smp value. If not, the sample should be diluted or a more concentrated ATP standard used

Products available online

Product		use
	ATP kit SL	Enzymes and metabolites
	Cellular ATP kit HTS	Enzymes and metabolites Cell proliferation/Cytotoxicity
	Cell viability kit SL	Cell proliferation/Cytotoxicity
	Intracellular ATP kit	Rapid microbiology
	Microbial ATP Kit	Rapid microbiology
	ATP Hygiene kit HS	Hygiene control
	ATP Biomass kit HS	Rapid microbiology
	Luciferase assay kit	Immunoassays
	Kinase RR kit	Kinase inhibition detection