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Home > ATP detection

 

ATP detection

The core technology of BioThema AB is analytical applications of the luminescent reaction of the Firefly Luciferase enzyme.
We provide luminescence assay reagents for monitoring of the Luciferase enzyme and ATP.

The standard in luminescence technology

BioThema has a unique ATP Standard that is stabilized in liquid to ensure accuracy of each luminometer reading. Calibration with internal Standard ATP accounts for variations between luminometers, inhibition or quenching of light from the sample and sensitivity of reagents.

ATP assay with ATP Reagent HS and Internal ATP Standard

  1. Add 0.1 ml extractant B/S to a cuvette.
  2. Add 0.1 ml sample to the cuvette.
  3. Add 0.4 ml reconstituted ATP Reagent HS and measure light emission, Ismp.
  4. Add 0.01 ml 10-7 mol/l ATP Standard, i.e. 1 pmol ATP, and measure light emission, Ismp+std.

Calculations

Calculate amount of ATP (pmol) in the sample by the equation:

ATPsmp = 1 * Ismp / ( Ismp + std - Ismp)

The factor 1 is the amount of ATP Standard added (i.e. 1 pmol).

Notes

  1. The higher the total assay volume the higher the sensitivity of the assay and the more accurate the results
  2. The (Ismp + std - Ismp) value should be at least 10x higher than the Ismp value. If not, the sample should be diluted or a more concentrated ATP standard used

Products available online

ATP Kit SL 1000 tests144-041
Cellular ATP Kit HTS 1200 tests155-050
Cell Viability Kit SL 1600 tests188-441
ATP Hygiene Kit HS (dropper bottles) 100 tests244-441
Intracellular ATP Kit HS 375 tests266-111
Microbial ATP Kit HS 375 tests266-112
ATP Biomass Kit HS266-311
Luciferase Assay Kit 800 tests484-001
Luciferase Assay Kit HTS 4800 tests for luminometers without dispensers484-102
Luciferase Assay Kit HTS 4600 tests for dispensing luminometers484-103

Product

use

ATP kit SL

Enzymes and metabolites

Cellular ATP kit HTS

Enzymes and metabolites
Cell proliferation/Cytotoxicity

Cell viability kit SL

Cell proliferation/Cytotoxicity

Intracellular ATP kit

Rapid microbiology

Microbial ATP Kit

Rapid microbiology

ATP Hygiene kit HS

Hygiene control

ATP Biomass kit HS

Rapid microbiology

Luciferase assay kit

Immunoassays

Kinase RR kit

Kinase inhibition detection

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