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CEInfinite

CEInfinite

The CEInfinite instrument is a new generation Imaging Detection CIEF system:

  • Whole column imaging detection based CIEF instrument for high throughput and reliable protein separation
  • High performance CMOS imaging sensor warrants overall system compactness, energy saving and robustness
  • World-class high voltage power supply ensures reproducible results
  • Digitalized with 14 bit ADC to improve overall detection dynamic range
  • Long-life deep UV LED light source to reduce replacement frequency
  • Automatic analysis is achieved with integration of state-of-the-art autosampler for fast injection and wash cycles
  • CEInsight CFR control and quantitation software provides a user friendly interface, reliable operation control, highly efficient data processing, and FDA title 21 CFR Part 11 compliance
  • CE and FCC certified to guarantee safe usage

The CEInfinite overcomes low analytical throughput and difficult method development associated with the long capillary of traditional single point detection CE. With a high sensitivity CMOS imaging sensor and powerful deep UV LED light source, CEInfinite dramatically increases the scan rate over 100 times compared to any other imaging CIEF systems. An increased scan rate allows study of fast dynamic processes more accurately, empowering Taylor dispersion measurement of protein hydrodynamic radius, protein interaction studies, and high resolution CIEF fractionation.

Detection Sensitivity Improvement

Limited by the small inner diameter of the separation capillary, there is always an unmet need of higher absorbance detection sensitivity in CE. Detection sensitivity of an imaging detection CIEF depends on both the detection wavelength and inner diameter of the separation column. A customized 214 nm imaging detection CIEF system with higher detection sensitivity (about 5 folds improvement over detection at 280 nm) could be implemented. We have also developed proprietary 200 µm ID separation cartridges, which provide a 100% sensitivity improvement over 100 µm ID ones.

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WCID CIEF Separation Resolution Improvement

Good protein separation resolution is always expected for CIEF, since isoelectric focusing is regarded to be a high resolution protein separation tool.

The separation resolution of a whole column detection imaging CIEF system relies on both the resolving power of the detection subsystem and resolving power of carrier ampholytes. The highest performance of a detection subsystem has been achieved with CEInfinite by design and selection of a high resolution optical imaging lens and high resolution high performance imaging sensor. Aeslytes, carrier ampholytes specially developed and synthesized for CIEF, possess qualities such as high resolving power, linear pH gradient, low protein interaction, low UV absorbance and fast focusing. The ampholytes are highly effective and have consistent quality.

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Breakthrough Technology of Coupling Scanning CIEF to MS

AES has developed proprietary technology that connects the platform straight to a mass spectrometer (patent pending). Protein CIEF separation and mobilization are monitored with real time scanning of the whole separation capillary, so that high resolution separation of proteins is ensured during focusing and transportation. Complex proteins are separated first according to their isoelectric point in our uniquely built cartridge and then the separated proteins are forced into the transfer capillary that is connected to the ion source of the mass spectrometer for protein characterization.

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More information about Carrier Ampholytes and pI markers here

CE - Prince technologies

To answer specific needs of today's scientific community, Prince Technologies introduces the PrinCE Capillary Electrophoresis System. The truly modular approach of the PrinCE System enables easy connection to any existing detector techniques (i.e. UV-VIS, DAD, LIF, Fluorescence, Conductivity, MS), but also rapid and easy implementation of future hardware developments such as specific CE detectors, CE-MS interfaces, sample preparation devices and fibre optics technology, whilst guaranteeing flexibility and upgrading to future needs without outdating existing equipment.

Prince Technologies offers a full range of:

  • CE systems
  • Detectors
  • Software
  • Buffers and kits
  • Consumables
  • Accessories